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M9490476.TXT
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1994-09-19
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Document 0476
DOCN M9490476
TI Preparative scale culture of Escherichia coli cells expressing the human
immunodeficiency virus type 1 Tat protein.
DT 9411
AU McKenna MC; Muchardt C; Gaynor R; Eisenberg D; Molecular Biology
Institute, University of California at Los; Angeles 90024.
SO Protein Expr Purif. 1994 Apr;5(2):105-11. Unique Identifier : AIDSLINE
MED/94331968
AB A procedure leading to a 100-liter fermentor culture of Escherichia coli
cells expressing the human immunodeficiency virus type 1 (HIV-1)
trans-activator (Tat) protein is described. The effects of growth
temperature and of cell density at the time of induction on the yield of
Tat were investigated. Tat was identified by SDS-gel electrophoresis and
Western blot. Tat represents approximately 10% of the soluble protein in
the cell lysate.
DE Amino Acid Sequence Escherichia coli/*GROWTH &
DEVELOPMENT/GENETICS/METABOLISM Fermentation Gene Products,
tat/BIOSYNTHESIS/*GENETICS/ISOLATION & PURIF Genes, tat Genes,
Synthetic HIV-1/*GENETICS Industrial Microbiology/*METHODS Molecular
Sequence Data Recombinant Fusion
Proteins/BIOSYNTHESIS/*GENETICS/ISOLATION & PURIF Support, U.S. Gov't,
P.H.S. JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).